Quantitative Synthetic Coxiella burnetii DNA can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load. Preparation includes fragments from com1, icd, transposase (IS1111A), gyrA, and sodB regions.